
After being snubbed for a promotion, Caroline Flint, the Europe Minister, delivered the most personal attack yet on the beleaguered Prime Minister, accusing him of using her as "female window dressing" and of operating a "two-tier Government".
In a move designed to maximise the damage to Mr Brown, she announced she was quitting his Government just as he faced the cameras to insist he would not walk away from Downing Street following Labour's worst performance at the polls in 30 years.
Less than 24 hours earlier, she had taken to the airwaves to support his leadership in the wake of the shock resignation of James Purnell, the Work and Pensions Secretary, and his demand that the Prime Minister go for the good of the party.
But her departure was just one of a series of ministerial walk-outs which undermined Mr Brown's hurried attempt to reshape his Government and relaunch his premiership.
Geoff Hoon, John Hutton, Paul Murphy, Margaret Beckett and Tony McNulty have all quit the Government.
They followed Jacqui Smith, Hazel Blears, Mr Purnell and Beverley Hughes out of the exit door.
Mr Brown was forced to retreat from his plan to sack Alistair Darling, the Chancellor, instead keeping him and Foreign Secretary David Miliband in their posts, promoting leadership rival Alan Johnson to the Home Office and effectively making Peter Mandelson deputy Prime Minister.
Miss Flint's resignation came after a stormy meeting at Downing Street.
She issued a savage resignation letter personally attacking Mr Brown's style of Government and the overwhelming pre-eminence of men.
"You have a two-tier Government, your inner circle and then the remainder of Cabinet," she said.
"Several of the women attending Cabinet – myself included – have been treated by you as little more than female window dressing."